LOCUS       sequence-66728-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX Zap70(C)-SH2
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..23
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(437..459)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(604..622)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        690..794
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggatctggt tccgcgtgga tccatggcta cgacggccca cgagcggatg ccctggtacc
       61 acagcagcct gacgcgtgag gaggccgagc gcaaacttta ctctggggcg cagaccgacg
      121 gcaagttcct gctgaggccg cggaaggagc agggcacata cgccctgtcc ctcatctatg
      181 ggaagacggt gtaccactac ctcatcagcc aagacaaggc gggcaagtac tgcattcccg
      241 agggcaccaa gtttgacacg ctctggcagc tggtggagta tctgaagctg aaggcggacg
      301 ggctcatcta ctgcctgaag gaggcctgcc ccaacagcag tgccagcaac gcctcagggg
      361 ctgctgctgg atccccggga attcatcgtg actgactgac gatctgcctc gcgcgtttcg
      421 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
      481 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
      541 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtataattct tgaagacgaa
      601 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      661 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      721 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      781 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      841 catttgctt
//
