LOCUS       sequence-66731-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX Zap70(N)-SH2
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..22
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(439..461)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(606..624)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        692..796
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatctggtt ccgcgtggat ccatgccaga ccccgcggcg cacctgccct tcttctacgg
       61 cagcatctcg cgtgccgagg ccgaggagca cctgaagctg gcgggcatgg cggacgggct
      121 cttcctgctg cgccagtgcc tgcgctcgct gggcggctat gtgctgtcgc tcgtgcacga
      181 tgtgcgcttc caccactttc ccatcgagcg ccagctcaac ggcacctacg ccattgccgg
      241 cggcaaagcg cactgtggac cggcagagct ctgcgagttc tactcgcgcg accccgacgg
      301 gctgccctgc aacctgcgca agccgtgcaa ccggccgtcg ggcctcgagc cgcagccggg
      361 ggtcttcgac ggatccccgg gaattcatcg tgactgactg acgatctgcc tcgcgcgttt
      421 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
      481 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
      541 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtataatt cttgaagacg
      601 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      661 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      721 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      781 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      841 ggcattttgc cttcctg
//
