LOCUS       sequence-66739-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX Grb2-SH2
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(383..405)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(550..568)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        636..740
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tggttccgcg tggatcccat ccgtggtttt ttggcaaaat ccccagagcc aaggcagaag
       61 aaatgcttag caaacagcgg cacgatgggg cctttcttat ccgagagagt gagagcgctc
      121 ctggggactt ctccctctct gtcaagtttg gaaacgatgt gcagcacttc aaggtgctcc
      181 gagatggagc cgggaagtac ttcctctggg tggtgaagtt caattctttg aatgagctgg
      241 tggattatca cagatctaca tctgtctcca gaaaccagca gatattcctg cgggacatag
      301 aacaggtgcc acagcagccg acatgaattc atcgtgactg actgacgatc tgcctcgcgc
      361 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
      421 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
      481 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat aattcttgaa
      541 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      601 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      661 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      721 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      781 ttgcggcatt ttgccttcct gtttttgctc acccagaaa
//
