LOCUS       sequence-66746-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX Mist-SH2 RK
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             478..522
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(586..608)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(753..771)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggatctggag ttctgttcca ggggcccctg ggatccgatg agaaggatgt ctggcagaat
       61 gaatggtaca ttggagaata cagtcgccag gcagtggaag atgtgttaat gaaagagaac
      121 aaggatggta cttttttggt taaagactgc tctacaaaat ccaaggcaga accatatgtt
      181 ttggtggtgt tttatgggaa caaggtctac aatgtgaaaa tccgtttcct cgagagcaat
      241 caacagtttg ccctgggcac aggactacga ggaaatgaga tgtttgattc tgtggaagac
      301 atcattgaac actacacata ttttcccatt ctgctaatag atgggaaaga caaggctgca
      361 cgcaggaaac agtgctacct cacccagcca ctgcctctcg ccaggctcct tctcactcag
      421 tactccagcc aggcacttca tgagggaatt catcgtgacg caagcttgct tggtggcggt
      481 ctgaacgaca tcttcgaggc tcagaaaatc gaatggcacg aataagcggc cgcatcgtga
      541 ctgactgacg atctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
      601 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
      661 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
      721 tagcggagtg tataattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
      781 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaa
//
