LOCUS       sequence-66756-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX Nck2-SH2
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             391..435
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(499..521)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(666..684)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggatctggan ttctgttcca ggggcccctg ggatccccct cgtccagcgg gcgcttcgcg
       61 ggcagagagt ggtactacgg gaacgtgacg cggcaccagg ccgagtgcgc cctcaacgag
      121 cggggcgtgg agggcgactt cctcattagg gacagcgagt cctcgcccag cgacttctcc
      181 gtgtccctta aagcgtcagg gaagaacaaa cacttcaagg tgcagctcgt ggacaatgtc
      241 tactgcattg ggcagcggcg cttccacacc atggacgagc tggtggaaca ctacaaaaag
      301 gcgcccatct tcaccagcga gcacggggag aagctctacc tcgtcagggc cctgcaggga
      361 attcatcgtg acgcaagctt gcttggtggc ggtctgaacg acatcttcga ggctcagaaa
      421 atcgaatggc acgaataagc ggccgcatcg tgactgactg acgatctgcc tcgcgcgttt
      481 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
      541 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
      601 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtataatt cttgaagacg
      661 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      721 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      781 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      841 ttgaaaaagg anag
//
