LOCUS       sequence-66760-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX SH2A-SH2
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..29
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             381..425
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(489..511)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(656..674)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        742..846
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggatctgga agttctgttc caggggcccc tgggatccca gaaaacctca gacaccatag
       61 ccccctggtt ccatggaatt ctcacactca agaaagcaaa tgaacttctt ctgagcacag
      121 gcatgcccgg cagttttctc atccgagtca gtgaaaggat caaaggctat gccctgtcct
      181 atctgtcgga ggacggctgt aaacatttcc tcatcgatgc ctctgcagac gcctacagct
      241 tcctgggcgt ggaccagcta cagcatgcca ccttggcgga tttggtggaa tatcacaagg
      301 aggaacccat cacttccctg gggaaggagc tccttctcta tccctgtggt cagcaggacc
      361 aggaaagctt gcttggtggc ggtctgaacg acatcttcga ggctcagaaa atcgaatggc
      421 acgaataagc ggccgcatcg tgactgactg acgatctgcc tcgcgcgttt cggtgatgac
      481 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
      541 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
      601 gccatgaccc agtcacgtag cgatagcgga gtgtataatt cttgaagacg aaagggcctc
      661 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
      721 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      781 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      841 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      901 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aa
//
