LOCUS       sequence-66812-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN backbone vector for use with the Golden Gate TALEN Kit in 
            place of pTAL1-4. Shorter N- and C-terminal tal protein segments 
            increases mutation induction; Fok I DD (improved 
            version=pCS2TAL3-DDD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Dahlem TJ, Hoshijima K, Jurynec MJ, Gunther D, Starker CG, Locke AS,
            Weis AM, Voytas DF, Grunwald DJ
  TITLE     Simple Methods for Generating and Detecting Locus-Specific Mutations
            Induced with TALENs in the Zebrafish Genome.
  JOURNAL   PLoS Genet. 2012 Aug;8(8):e1002861. Epub 2012 Aug 16.
  PUBMED    22916025
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2012-08"; volume: "8(8)"; pages: "e1002861. Epub 
            2012 Aug 16"
COMMENT     Sequence Label: pCS2TAL3-DD
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..630)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQD
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     protein_bind    816..837
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(852..857,858..875,876..882)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    890..906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     895..917
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     914..930
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     914..930
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgtctggatc tacgtaatac gactcactat agttctagat taaaagttta tctcgccgtt
       61 attaaatttc cggcgcactt cctctaaggt taatgtgccg gctttaatca tttctccacc
      121 aattaaaagc tcttctacac taagaacagc tccattacaa ttagtgatat gatttaatcg
      181 tgtaagctga gctttgtagt ttcctttaaa gtgaccactc acaaataaaa acttaaattc
      241 cgttacagaa gatggataga ctttccacca ttcattaggg ttgatatgtt tgtttcgtgt
      301 ttgattttct tcgacatagt cttgcatttc atctgcttgg ccaattggca gattataacc
      361 tccgctataa gctttagtat ccacgatcac accgtaatca ataggagatc cgacagtata
      421 aattgctccg tccggtttcc ttgatccacc caaatgttta cctctatatc cataaacttt
      481 cataaaaaat tccattacct tcatttcaag aattctatcc tgagtggaat ttctggcaat
      541 ttcaattaat tcaatatatt catgaggcac atatttcaat ttatgacgaa gttcagattt
      601 cttctcctcc agttcacttt tgactagttg ggatccggca acgcgatggg acgtgcgttc
      661 gccaatacgg cgattgactc ttctgatcaa ttccggcgcg tgcggcaatc cctttttcac
      721 tgcatccatg gcaggacgtc cgccgaggca ggccaaggcg acgaggtggt cgttggagac
      781 gaccggtgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
      841 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
      901 taacaatttc acacaggaaa cagctatgac catgattacg cca
//
