LOCUS       sequence-66815-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  TITLE     Craig lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b/EYFP-GgVcl 884-1066-ECFP FRET
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(155..157,158..160,161..673)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(200..221)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(461..480)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acaggcaact gagatgttgg ttcataacgc ccagaacctc atgcagtctg tgaaggaaac
       61 tgtgagagaa gctgaagcag catccattaa gataagaaca gatgccggat tcactctgcg
      121 ctgggtcaga aagaccccat ggtatcaggc tagcatggtg agcaagggcg aggagctgtt
      181 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      241 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      301 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt
      361 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      421 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      481 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      541 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca
      601 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
      661 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      721 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
      781 caaagacccc aacgagaagc gcgatcacat ggtccttgct gga
//
