LOCUS       sequence-66817-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Chen H, Cohen DM, Choudhury DM, Kioka N, Craig SW
  TITLE     Spatial distribution and functional significance of activated 
            vinculin in living cells.
  JOURNAL   J Cell Biol. 2005 May 9;169(3):459-70.
  PUBMED    15883197
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2005-05-9"; volume: "169(3)"; pages: "459-70"
COMMENT     Sequence Label: pET15b/Shigella flexneri IpaA
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..103
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca
       61 tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgc ataatgtaaa
      121 taatactcaa gcgccaacat tcttatataa ggcaacttca ccatcatcaa cagaatacag
      181 cgagttaaaa agcaaaatat ccgatatcca tagttcgcaa acttctctaa aaacaccagc
      241 atcagtgtct gaaaaagaaa actttgcaac gtcttttaat cagaaatgtc ttgatttttt
      301 attttcttcc tcagggaaag aagatgtgtt aagaagcatt tattccaact caatgaatgc
      361 gtatgccaaa agcgagattc tcgaattttc aaatgttttg tactccttag tacatcaaaa
      421 tggtcttaat tttgaaaacg aaaagggact tcaaaaaatt gtcgcacagt attcggaact
      481 aattataaaa gataaattat cccaagattc tgcctttgga ccatggtcgg caaagaataa
      541 gaaactccat caattacgac aaaacattga gcacagactt gcactattag cacaacaaca
      601 cacatctggt gaagctttat cattgggaca aaaactcctc aatactgaag tatcatcatt
      661 tatcaagaat aatattcttg ctgaattaaa gttaagtaat gaaactgttt catctctcaa
      721 actagatgat ttagttgacg cacaggcaaa acttgccttt gatagtttgc gcaatcaacg
      781 taaaaatact attgatagta aaggatttgg tataggtaaa ctgtcaagag acttaaatac
      841 agtagccgtg tttcctgagc tgttgagaaa agtccttaat gatattttag aaga
//
