LOCUS       sequence-66818-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Steimle PA, Hoffert JD, Adey NB, Craig SW
  TITLE     Polyphosphoinositides inhibit the interaction of vinculin with actin
            filaments.
  JOURNAL   J Biol Chem. 1999 Jun 25;274(26):18414-20.
  PUBMED    10373448
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1999-06-25"; volume: "274(26)"; pages: "18414-20"
COMMENT     Sequence Label: pET15b/GgVcl 884-1066 (aka Vt)
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..103
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(717..735)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      731..778
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca
       61 tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgg agttcccaga
      121 gcagaaagca ggagaagcta ttaatcagcc catgatgatg gctgctaggc agttgcatga
      181 cgaggctcgg aaatggtcta gcaagggtaa cgacatcatt gctgctgcta aacgaatggc
      241 gctgctcatg gctgagatgt cacgcctggt gcgaggaggc agcggaaaca agcgtgccct
      301 catccagtgt gcaaaagata ttgctaaggc atcggatgaa gtcactcgat tggccaaaga
      361 ggtggccaag caatgtactg ataaacgcat tagaacaaac ctcttacagg tctgtgagcg
      421 aatcccaacc atcagcacgc agctcaaaat tctttccaca gtgaaagcta ccatgctggg
      481 caggactaac atcagcgatg aagaatcaga acaggcaact gagatgttgg ttcataacgc
      541 ccagaacctc atgcagtctg tgaaggaaac tgtgagagaa gctgaagcag catccattaa
      601 gataagaaca gatgccggat tcactctgcg ctgggtcaga aagaccccat ggtatcagta
      661 gctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      721 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      781 gaaaggagga actatatccg gatatcccgc aagaggcccg gcagtaccgg cataaccaag
      841 cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat
      901 ttcata
//
