LOCUS       sequence-66819-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Mon H, Sugahara R, Hong SM, Lee JM, Kamachi Y, Kawaguchi Y, Kusakabe
            T
  TITLE     Analysis of protein interactions with two-hybrid system in cultured 
            insect cells.
  JOURNAL   Anal Biochem. 2009 Sep 15;392(2):180-2. doi: 
            10.1016/j.ab.2009.05.033.
  PUBMED    19481053
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2009.05"; journalName: "Anal Biochem"; date: 
            "2009-09-15- 15"; volume: "392"; issue: "2"; pages: "180-2"
COMMENT     Sequence Label: pIE2-DBD
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..180
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             208..648
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    649..773
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(798..803,804..821,822..828)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 gttcgaattt aaagcttggt accgagatcg aggcctgtct agagaagctc cgccaccatg
       61 gatctccacc gcggtggagg ccgcatcttt tacccatacg atgttcctga ctatgcgggc
      121 tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc agattacgct
      181 gctcatggcg gacaccggta tactagtatg aagctactgt cttctatcga acaagcatgc
      241 gatatttgcc gacttaaaaa gctcaagtgc tccaaagaaa aaccgaagtg cgccaagtgt
      301 ctgaagaaca actgggagtg tcgctactct cccaaaacca aaaggtctcc gctgactagg
      361 gcacatctga cagaagtgga atcaaggcta gaaagactgg aacagctatt tctactgatt
      421 tttcctcgag aagaccttga catgattttg aaaatggatt ctttacagga tataaaagca
      481 ttgttaacag gattatttgt acaagataat gtgaataaag atgccgtcac agatagattg
      541 gcttcagtgg agactgatat gcctctaaca ttgagacagc atagaataag tgcgacatca
      601 tcatcggaag agagtagtaa caaaggtcaa agacagttga ctgtatcgac aagtttgtac
      661 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
      721 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
      781 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
      841 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
      901 gatatacc
//
