LOCUS       sequence-66820-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Mon H, Sugahara R, Hong SM, Lee JM, Kamachi Y, Kawaguchi Y, Kusakabe
            T
  TITLE     Analysis of protein interactions with two-hybrid system in cultured 
            insect cells.
  JOURNAL   Anal Biochem. 2009 Sep 15;392(2):180-2. doi: 
            10.1016/j.ab.2009.05.033.
  PUBMED    19481053
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2009.05"; journalName: "Anal Biochem"; date: 
            "2009-09-15- 15"; volume: "392"; issue: "2"; pages: "180-2"
COMMENT     Sequence Label: pIE2-DBD
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    189..318
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     CDS             complement(336..353)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(363..404)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    434..558
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(701..720)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
       61 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      121 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      181 atcgatccca cgcgcttgaa aggagtgtgt aaatggacaa tgttgttaca ataaaagttg
      241 aaattatttt ataaaattta ttaaaaacat atttaaacat aacatattgt attaaaacat
      301 gacaatacaa actaagattt agtcagataa actcaatggt gatggtgatg atgaccggta
      361 cgcgtagaat cgagaccgag gagagggtta gggataggct taccttcgaa ccgcgggccc
      421 tctagcttac gtcaccactt tgtacaagaa agctgaacga gaaacgtaaa atgatataaa
      481 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
      541 acatatccag tcactatggt cgacctgcag actggctgtg tataagggag cctgacattt
      601 atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat
      661 cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc
      721 gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggggact ttatctgaca
      781 gcagacgtgc actggccagg gggatcacca tccgtcgccc gggc
//
