LOCUS       sequence-66826-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Cohen DM, Chen H, Johnson RP, Choudhury B, Craig SW
  TITLE     Two distinct head-tail interfaces cooperate to suppress activation 
            of vinculin by talin.
  JOURNAL   J Biol Chem. 2005 Apr 29;280(17):17109-17. Epub 2005 Feb 22.
  PUBMED    15728584
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-04-29"; volume: "280(17)"; pages: "17109-17. 
            Epub 2005 Feb 22"
COMMENT     Sequence Label: pET15b/GgVcl V1-258 (aka VD1)
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatgatg cccgtcttcc
      121 acacgcgcac catcgagagc atcttggagc ccgtggctca gcagatctcc cacctggtca
      181 tcatgcacga ggagggggag gtagacggca aggccatccc ggacctcacc gcccccgtgt
      241 cggccgtgca ggccgctgtc agcaacctgg tgcgggttgg aaaagaaact gtgcagacaa
      301 cagaagacca gatcttgaaa agggatatgc caccagcatt catcaaagta gagaatgcct
      361 gcaccaagct cgttcgagca gcccagatgc tgcaagcaga tccttattca gtaccagctc
      421 gtgactacct aattgatgga tcaagaggca tcctttctgg aacatcagac ttacttctga
      481 catttgatga agcggaggtc cgtaaaatca tccgtgtctg caaaggaata ttggaatatc
      541 tgactgtggc agaagtagta gagactatgg aggatttggt gacatataca aagaatctag
      601 ggccaggaat gacaaagatg gcgaaaatga tcgatgagag acaacaggaa ttaactcatc
      661 aggaacatag ggttatgctg gtgaactcca tgaatactgt gaaggagcta ttgcctgtac
      721 ttatttcagc tatgaagatc tttgtaacca caaaaaacac taaaagccag ggaatagaag
      781 aggccttgaa aaatcgcaat ttcacagtag agaaaatgag tgctgagata aatgaaataa
      841 tccgtgtatt acaactcact tcctgggatg aagatgcctg gt
//
