LOCUS       sequence-66836-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     Craig lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b/GgVcl 884-1066-ECFP
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(702..723)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tagcatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatggag ttcccagagc
      121 agaaagcagg agaagctatt aatcagccca tgatgatggc tgctaggcag ttgcatgacg
      181 aggctcggaa atggtctagc aagggtaacg acatcattgc tgctgctaaa cgaatggcgc
      241 tgctcatggc tgagatgtca cgcctggtgc gaggaggcag cggaaacaag cgtgccctca
      301 tccagtgtgc aaaagatatt gctaaggcat cggatgaagt cactcgattg gccaaagagg
      361 tggccaagca atgtactgat aaacgcatta gaacaaacct cttacaggtc tgtgagcgaa
      421 tcccaaccat cagcacgcag ctcaaaattc tttccacagt gaaagctacc atgctgggca
      481 ggactaacat cagcgatgaa gaatcagaac aggcaactga gatgttggtt cataacgccc
      541 agaacctcat gcagtctgtg aaggaaactg tgagagaagc tgaagcagca tccattaaga
      601 taagaacaga tgccggattc actctgcgct gggtcagaaa gaccccatgg tatcagatgg
      661 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      721 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      781 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      841 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
      901 acgacttctt cagtcgcatg ccgacgaagg
//
