LOCUS       sequence-66837-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Craig lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b/GgVcl 884-1066-ECFP
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(74..92)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      88..135
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(310..315,316..332,333..338))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(365..383)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        451..555
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(774..793)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gacgagctgt acaagtaact cgaggatccg gctgctaaca aagcccgaaa ggaagctgag
       61 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      121 ttgaggggtt ttttgctgaa aggaggaact atatccggat atcccgcaag aggcccggca
      181 gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga
      241 tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata
      301 aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga
      361 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      421 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      481 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      541 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      601 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      661 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      721 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      781 ggcgcggtat tatcccgtgt ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
//
