LOCUS       sequence-66840-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CRY2(1-498)-mCherry fusion for use in light-inducible 
            protein interaction modules.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL
  TITLE     Rapid blue-light-mediated induction of protein interactions in 
            living cells.
  JOURNAL   Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524
  PUBMED    21037589
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2010-12"; volume: "7"; issue: 
            "12"; pages: "973-5"
COMMENT     Sequence Label: pCRY2PHR-mCherryN1
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(147..857)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(425..444)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     694..712
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      854..863
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
       61 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      121 gattatgatc tagagtcgcg gccgctttac ttgtacagct cgtccatgcc gccggtggag
      181 tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg
      241 atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg
      301 taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg
      361 atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc
      421 atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc
      481 ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg
      541 tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag
      601 tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac
      661 aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg
      721 gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg
      781 gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat gttatcctcc
      841 tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg ctgctgctcc gatcatgatc
      901 tgtgcttcac gggttcttga aatagcttta gctagtagct cacgagctgt gtcgatgtct
      961 acaatgggtt tcgca
//
