LOCUS       sequence-66849-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  TITLE     Craig lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p401F/GgVcl 1-1066 (N term FLAG tag)
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        240..258
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     240..257
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     271..290
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      335..344
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             344..367
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg
      121 cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag gcccaccccc
      181 ttggcttcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga
      241 tttaggtgac actatagaat aacatccact ttgcctttct ctccacaggt gtccactccc
      301 aggtccaact gcacctcggt tctatcgatc agccgccacc atggactaca aggacgacga
      361 tgacaaggga tccaaagaat tcgcccttca tatgatgccc gtcttccaca cgcgcaccat
      421 cgagagcatc ttggagcccg tggctcagca gatctcccac ctggtcatca tgcacgagga
      481 gggggaggta gacggcaagg ccatcccgga cctcaccgcc cccgtgtcgg ccgtgcaggc
      541 cgctgtcagc aacctggtgc gggttggaaa agaaactgtg cagacaacag aagaccagat
      601 cttgaaaagg gatatgccac cagcattcat caaagtagag aatgcctgca ccaagctcgt
      661 tcgagcagcc cagatgctgc aagcagatcc ttattcagta ccagctcgtg actacctaat
      721 tgatggatca agaggcatcc tttctggaac atcagactta cttctgacat ttgatgaagc
      781 ggaggtccgt aaaatcatcc gtgtctgcaa aggaatattg gaatatctga ctgtggcaga
      841 agtagtagag actatggagg atttggtgac atatacaaag aatctagggc caggaatgac
      901 aaagatg
//
