LOCUS       sequence-66852-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Lu J, Beatty L, Pinthus J
  TITLE     Dual expression recombinase based (DERB) single vector system for 
            high throughput screening and verification of protein interactions 
            in living cells
  JOURNAL   Nat Prec (2008).
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Prec 
            (2008)."
COMMENT     Sequence Label: Y pFe-YcLoxP-YnAtt
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(131..255)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(316..335)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(317..335)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(346..363)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(346..362)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(355..377)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    403..524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(414..433)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     468..487
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
       61 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
      121 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
      181 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
      241 cttgtacaaa gtggttgatg ggcggccgct ctagagggcc caagcttacg cgtgcatgcg
      301 acgtcatagc tctctcccta tagtgagtcg tattataagc taggcactgg ccgtcgtttt
      361 acaacgtcgt gactgggaaa actgctagct ggccagacat gataagatac attgatgagt
      421 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      481 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      541 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      601 tctacaaatg tggtatggaa atgttaatta actagccatg accaaaatcc cttaacgtga
      661 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      721 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      781 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
      841 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
      901 tgtagcaccg cct
//
