LOCUS       sequence-66865-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Spitzer JI, Ugras S, Runge S, Decarolis P, Antonescu C, Tuschl T, 
            Singer S
  TITLE     mRNA and protein levels of FUS, EWSR1, and TAF15 are upregulated in 
            liposarcoma.
  JOURNAL   Genes Chromosomes Cancer. 2011 May . 50(5):338-47.
  PUBMED    21344536
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Chromosomes Cancer. 2011 May . 50(5):338-47."
COMMENT     Sequence Label: pFRT_DESTFLAGHA
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..284)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(300..326)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(327..350)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      350..359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(423..442)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(424..442)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(487..690)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(516..536)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
      181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      241 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgttgatat ctggttcaaa
      301 gcgtaatcgg gcacgtcata agggtacttg tcatcgtcgt ccttgtagtc catggcggcg
      361 gcgatcagaa ttccaccaca ctatcgatgg atcgatccga gctcgctagc cagcttgggt
      421 ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag
      481 ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      541 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
      601 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
      661 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
      721 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      781 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
      841 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
      901 cgttactatg ggaacatacg tcattattga c
//
