LOCUS       sequence-66876-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Spitzer JI, Ugras S, Runge S, Decarolis P, Antonescu C, Tuschl T, 
            Singer S
  TITLE     mRNA and protein levels of FUS, EWSR1, and TAF15 are upregulated in 
            liposarcoma.
  JOURNAL   Genes Chromosomes Cancer. 2011 May . 50(5):338-47.
  PUBMED    21344536
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Chromosomes Cancer. 2011 May . 50(5):338-47."
COMMENT     Sequence Label: pFRT_TO_DESTFLAGHA
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(106..112,113..130,131..136))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(161..285)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(301..327)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(328..351)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(451..475)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    485..503
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    506..524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(526..729)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(555..575)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
       61 ccttagctcc tgaaaatctc gacggatcct aactcaaaat ccacacatta tacgagccgg
      121 aagcataaag tgtaaagcct ggggtgccta atgcggccgc catagtgact ggatatgttg
      181 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      241 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgttgata tctggttcaa
      301 agcgtaatcg ggcacgtcat aagggtactt gtcatcgtcg tccttgtagt ccatggtacc
      361 aagcttaagt ttaaacgcta gagtccggag gctggatcgg tcccggtgtc ttctatggag
      421 gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctcgtcg
      481 acgatctcta tcactgatag ggagatctct atcactgata gggagagctc tgcttatata
      541 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
      601 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg
      661 gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa
      721 ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
      781 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
      841 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
      901 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
      961 cattattg
//
