LOCUS       sequence-66884-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMAD-Gate1
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             125..145
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    337..461
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(486..491,492..509,510..516)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(636..655)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtccactccc agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag
       61 gctagccagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta tcgaacaagc
      121 atgcccaaaa aagaagagaa aggtagatga attcccgggg atctcgacgg cccccccgac
      181 cgatgtcagc ctgggggacg agctccactt agacggcgag gacgtggcga tggcgcatgc
      241 cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg
      301 atcgccaggg atccgtcgac ttgacgcgtt gatatcacaa gtttgtacaa aaaagctgaa
      361 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
      421 actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc
      481 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt
      541 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
      601 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
      661 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
      721 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
      781 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
      841 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
      901 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
      961 tggccta
//
