LOCUS       sequence-66886-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMAD-Gate2
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             128..148
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    341..465
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(490..495,496..513,514..520)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(640..659)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
       61 taggctagcc agcttgaagc aagcctcctg aaagatgaag ctactgtctt ctatcgaaca
      121 agcatgccca aaaaagaaga gaaaggtaga tgaattcccg gggatctcga cggccccccc
      181 gaccgatgtc agcctggggg acgagctcca cttagacggc gaggacgtgg cgatggcgca
      241 tgccgacgcg ctagacgatt tcgatctgga catgttgggg gacggggatt ccccgggtcc
      301 gggatcgcca gggatccgtc gacttgacgc gttgatatca acaagtttgt acaaaaaagc
      361 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
      421 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
      481 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
      541 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
      601 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
      661 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
      721 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
      781 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
      841 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
      901 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
      961 aacctggcct atttccctaa agggtttatt g
//
