LOCUS       sequence-66898-e        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Chen H, Cohen DM, Choudhury DM, Kioka N, Craig SW
  TITLE     Spatial distribution and functional significance of activated 
            vinculin in living cells.
  JOURNAL   J Cell Biol. 2005 May 9;169(3):459-70.
  PUBMED    15883197
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2005-05-9"; volume: "169(3)"; pages: "459-70"
COMMENT     Sequence Label: GgVcl 1-1066 FRET tail probe 1
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(164..166,167..169,170..883)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(209..230)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(470..489)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     817..838
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agaacaggca actgagatgt tggttcataa cgcccagaac ctcatgcagt ctgtgaagga
       61 aactgtgaga gaagctgaag cagcatccat taagataaga acagatgccg gattcactct
      121 gcgctgggtc agaaagaccc catggtatca ggtcgacggt accatggtga gcaagggcga
      181 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      241 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      301 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
      361 ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
      421 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      481 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      541 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      601 catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
      661 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      721 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
      781 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      841 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag
      901 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
      961 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgtta
//
