LOCUS       sequence-66899-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMAD-Gate5
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..284)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    369..490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(380..399)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     434..453
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(711..730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
      181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      241 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgttgatat catctagagc
      301 ggccgcaggt acctgaataa ctaaggccgc ttccctttag tgagggttaa tgcttcgagc
      361 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
      421 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
      481 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
      541 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatccgat aaggatcgat
      601 tccggagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      661 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      721 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      781 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
      841 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
      901 ggagtccacg t
//
