LOCUS       sequence-66900-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Dagliyan O, Shirvanyants D, Karginov AV, Ding F, Fee L, 
            Chandrasekaran SN, Freisinger CM, Smolen GA, Huttenlocher A, Hahn 
            KM, Dokholyan NV
  TITLE     Rational design of a ligand-controlled protein conformational 
            switch.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6800-4. doi: 
            10.1073/pnas.1218319110. Epub 2013 Apr 8.
  PUBMED    23569285
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1218319110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: "6800-4"
COMMENT     Sequence Label: Tol2 Krt4 Src (YF)-uniRapR-cerulean
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(306..335)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(348..599)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(390..411)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     739..758
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 taatacgact cactatagtt ctagagtcga ctgcagaatt ctgcagatca tggcaggcat
       61 gggacagtca ctgtaccagc ctccacctgt ccttccaggc tgccccatcc ctgaagccct
      121 tccatgctcc aaaaggggca ttgccgaggc aacgcagccc cagagagagg atgaagggag
      181 gcaggtgcat cggaggcaac acccggaagc ctagaggtga cagttctgcg cacacagtcc
      241 ctataggttc tccccgggct ggtactgtgg ctctagaggt gacagttctg cgcacacagt
      301 cctcacaggt cctcctcgga gatcagcttc tgctccccgc ttccggactt gtacagctcg
      361 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
      421 ttctcgttgg ggtctttgct cagcttggac tgggtgctca ggtagtggtt gtcgggcagc
      481 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
      541 ccgtcctcga tgttgtggcg gatcttgaag ttggccttga tgccgttctt ctgcttgtcg
      601 gcggtgatat agacgttgtc gctgatggcg ttgtactcca gcttgtgccc caggatgttg
      661 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
      721 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
      781 tggacgtagc cttcgggcat ggcgga
//
