LOCUS       sequence-66916-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMBD-Gate1
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    45..169
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(194..199,200..217,218..224)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             278..937
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(344..363)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcgccggaat tcccggggat ccgtcgactt gacgcgttga tatcacaagt ttgtacaaaa
       61 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
      121 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt
      181 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta
      241 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
      301 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
      361 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
      421 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
      481 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
      541 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
      601 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
      661 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
      721 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
      781 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
      841 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
      901 acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa
      961 agccagataa ca
//
