LOCUS       sequence-66918-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMBD-Gate1
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(133..257)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    341..462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(352..371)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     406..425
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(735..754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     945..966
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
       61 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      121 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
      181 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
      241 ttcttgtaca aagtggtgat atcatctaga gcggccgcag gtacctgaat aactaaggcc
      301 gcttcccttt agtgagggtt aatgcttcga gcagacatga taagatacat tgatgagttt
      361 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
      421 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
      481 cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc
      541 tacaaatgtg gtaaaatccg ataaggatcg atccgggctg gcgtaatagc gaagaggccc
      601 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag
      661 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      721 cgccctagcg cccgctcctt tcgctttctt cccttccttt cacgccacgt tcgccggctt
      781 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      841 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
      901 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
      961 aactggaaca acactcaacc ctatctcggt ctatt
//
