LOCUS       sequence-66919-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMBD-Gate2
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..535
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    578..702
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(727..732,733..750,751..757)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(877..896)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
       61 taggctagcc agcttgaagc aagcctcctg aaagatgaag ctactgtctt ctatcgaaca
      121 agcatgcgat atttgccgac ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc
      181 caagtgtctg aagaacaact gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct
      241 gactagggca catctgacag aagtggaatc aaggctagaa agactggaac agctatttct
      301 actgattttt cctcgagaag accttgacat gattttgaaa atggattctt tacaggatat
      361 aaaagcattg ttaacaggat tatttgtaca agataatgtg aataaagatg ccgtcacaga
      421 tagattggct tcagtggaga ctgatatgcc tctaacattg agacagcata gaataagtgc
      481 gacatcatca tcggaagaga gtagtaacaa aggtcaaaga cagttgactg tatcgccgga
      541 attcccgggg atccgtcgac ttgacgcgtt gatatcaaca agtttgtaca aaaaagctga
      601 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      661 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc
      721 ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg
      781 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
      841 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
      901 tgtacctata accagaccgt tcagctggat attacggcct tttta
//
