LOCUS       sequence-66921-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Chen H, Cohen DM, Choudhury DM, Kioka N, Craig SW
  TITLE     Spatial distribution and functional significance of activated 
            vinculin in living cells.
  JOURNAL   J Cell Biol. 2005 May 9;169(3):459-70.
  PUBMED    15883197
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2005-05-9"; volume: "169(3)"; pages: "459-70"
COMMENT     Sequence Label: pEYFPC1/GgVcl 1-1066 (YFP at N term)
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(54..56,57..59,60..770)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(99..120)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(360..379)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     707..728
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga
       61 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      121 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      181 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      241 ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
      301 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      361 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      421 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      481 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
      541 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
      601 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
      661 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
      721 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca
      781 gatctcgagc tcaagcttcg aattccggcc ccccctgccc cgccgccgcc atgcccgtct
      841 tccacacgcg cac
//
