LOCUS       sequence-6693-ex        5946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Tran H, Brunet A, Grenier JM, Datta SR, Fornace AJ Jr, DiStefano PS,
            Chiang LW, Greenberg ME
  TITLE     DNA repair pathway stimulated by the forkhead transcription factor 
            FOXO3a through the Gadd45 protein.
  JOURNAL   Science 2002 Apr 19;296(5567):530-4.
  PUBMED    11964479
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2002-04-19"; volume: "296(5567)"; pages: "530-4"
COMMENT     Sequence Label: HA-FOXO3a WT-ER
FEATURES             Location/Qualifiers
     source          1..5946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    115..250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(153..172)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     207..226
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(309..328)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(587..604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(758..1346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(838..857)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1518..2309,2310..2378))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2141..2160
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2379..2483)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2513..2870
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2513..2704
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2536..2556)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2721..2856
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2895..2921
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     4958..4974
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4958..4974
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 taagtaatga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
       61 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      121 tttattgcag gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
      241 tgtctggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
      301 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
      361 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
      421 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
      481 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtgcggtgt gaaataccgc
      541 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
      601 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      661 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      721 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      781 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      841 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      901 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
      961 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1021 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1081 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1141 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     1201 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1261 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1321 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1381 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1441 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaaag tatatatgag
     1501 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1561 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1621 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1681 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1741 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1801 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     1861 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     1921 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1981 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2041 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2101 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     2161 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2221 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2281 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2341 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2401 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2461 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc ttctgaggcg
     2521 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     2581 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     2641 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     2701 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2761 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2821 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa agatctaagc
     2881 ttgccgccac catgtatgat gttcctgatt atgctagcct cccggggaat tcggcagagg
     2941 caccggcttc cccggccccg ctctctccgc tcgaagtgga gctggacccg gagttcgagc
     3001 cccagagccg tccgcgatcc tgtacgtggc ccctgcaaag gccggagctc caagcgagcc
     3061 ctgccaagcc ctcgggggag acggccgctg actccatgat ccccgaggag gaggacgatg
     3121 aagacgacga ggacggcggg ggacgggccg gctcggccat ggcgatcggc ggcggcggcg
     3181 ggagcggcac gctgggctcc gggctgctcc ttgaggactc ggcccgggtg ctggcacccg
     3241 gagggcaaga ccccgggtct gggccagcca ccgcggcggg cgggctgagc gggggtacac
     3301 aggcgctgct gcagcctcag caaccgctgc caccgccgca gccgggggcg gctgggggct
     3361 ccgggcagcc gaggaaatgt tcgtcgcggc ggaacgcctg gggaaacctg tcctacgcgg
     3421 acctgatcac ccgcgccatc gagagctccc cggacaaacg gctcactctg tcccagatct
     3481 acgagtggat ggtgcgttgc gtgccctact tcaaggataa gggcgacagc aacagctctg
     3541 ccggctggaa gaactccatc cggcacaacc tgtcactgca tagtcgattc atgcgggtcc
     3601 agaatgaggg aactggcaag agctcttggt ggatcatcaa ccctgatggg gggaagagcg
     3661 gaaaagcccc ccggcggcgg gctgtctcca tggacaatag caacaagtat accaagagcc
     3721 gtggccgcgc agccaagaag aaggcagccc tgcagacagc ccccgaatca gctgacgaca
     3781 gtccctccca gctctccaag tggcctggca gccccacgtc acgcagcagt gatgagctgg
     3841 atgcgtggac ggacttccgt tcacgcacca attctaacgc cagcacagtc agtggccgcc
     3901 tgtcgcccat catggcaagc acagagttgg atgaagtcca ggacgatgat gcgcctctct
     3961 cgcccatgct ctacagcagc tcagccagcc tgtcaccttc agtaagcaag ccgtgcacgg
     4021 tggaactgcc acggctgact gatatggcag gcaccatgaa tctgaatgat gggctgactg
     4081 aaaacctcat ggacgacctg ctggataaca tcacgctccc gccatcccag ccatcgccca
     4141 ctgggggact catgcagcgg agctctagct tcccgtatac caccaagggc tcgggcctgg
     4201 gctccccaac cagctccttt aacagcacgg tgttcggacc ttcatctctg aactccctac
     4261 gccagtctcc catgcagacc atccaagaga acaagccagc taccttctct tccatgtcac
     4321 actatggtaa ccagacactc caggacctgc tcacttcgga ctcacttagc cacagcgatg
     4381 tcatgatgac acagtcggac cccttgatgt ctcaggccag caccgctgtg tctgcccaga
     4441 attcccgccg gaacgtgatg cttcgcaatg atccgatgat gtcctttgct gcccagccta
     4501 accagggaag tttggtcaat cagaacttgc tccaccacca gcaccaaacc cagggcgctc
     4561 ttggtggcag ccgtgccttg tcgaattctg tcagcaacat gggcttgagt gagtccagca
     4621 gccttgggtc agccaaacac cagcagcagt ctcctgtcag ccagtctatg caaaccctct
     4681 cggactctct ctcaggctcc tccttgtact caactagtgc aaacctgccc gtcatgggcc
     4741 atgagaagtt ccccagcgac ttggacctgg acatgttcaa tgggagcttg gaatgtgaca
     4801 tggagtccat tatccgtagt gaactcatgg atgctgatgg gttggatttt aactttgatt
     4861 ccctcatctc cacacagaat gttgttggtt tgaacgtggg gaacttcact ggtgctaagc
     4921 aggcctcatc tcagagctgg gtgccaggcg cggccgctct agaactagtg gatccacgaa
     4981 atgaaatggg tgcttcagga gacatgaggg ctgccaacct ttggccaagc cctcttgtga
     5041 ttaagcacac taagaagaat agccctgcct tgtccttgac agctgaccag atggtcagtg
     5101 ccttgttgga tgctgaaccg cccatgatct attctgaata tgatccttct agacccttca
     5161 gtgaagcctc aatgatgggc ttattgacca acctagcaga tagggagctg gttcatatga
     5221 tcaactgggc aaagagagtg ccaggctttg gggacttgaa tctccatgat caggtccacc
     5281 ttctcgagtg tgcctggctg gagattctga tgattggtct cgtctggcgc tccatggaac
     5341 acccggggaa gctcctgttt gctcctaact tgctcctgga caggaatcaa ggtaaatgtg
     5401 tggaaggcat ggtggagatc tttgacatgt tgcttgctac gtcaagtcgg tcccgcatga
     5461 tgaacctgca gggtgaagag tttgtgtgcc tcaaatccat cattttgctt aattccggag
     5521 tgtacacgtt tctgtccagc accttgaagt ctctggaaga gaaggaccac atccaccgtg
     5581 tcctggacaa gatcacagac actttgatcc acctgatggc caaagctggc ctgactctgc
     5641 agcagcagca tcgccgccta gctcagctcc ttctcattct ttcccatatc cggcacatga
     5701 gtaacaaacg catggagcat ctctacaaca tgaaatgcaa gaacgtggta cccctctatg
     5761 acctgctcct ggagatgttg gatgcccacc gccttcatgc cccagccagt cgcatgggag
     5821 tgcccccaga ggagcccagc cagacccagc tggccaccac cagctccact tcagcacatt
     5881 ccttacaaac ctactacata cccccggaag cagagggctt ccccaacacg atctgagagc
     5941 tctaga
//
