LOCUS       sequence-66935-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMBD-Gate3
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..536
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    580..704
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(729..734,735..752,753..759)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(879..898)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
       61 ataggctagc cagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac
      121 aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg
      181 ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc
      241 tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc
      301 tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata
      361 taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag
      421 atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg
      481 cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg
      541 aattcccggg gatccgtcga cttgacgcgt tgatatcaaa caagtttgta caaaaaagct
      601 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
      661 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
      721 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
      781 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
      841 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
      901 aatgtaccta taaccagacc gt
//
