LOCUS       sequence-66936-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMBD-Gate3
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    50..174
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(199..204,205..222,223..229)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(349..368)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtatcgccgg aattcccggg gatccgtcga cttgacgcgt tgatatcaaa caagtttgta
       61 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
      121 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc
      181 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      241 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      301 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      361 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      421 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      481 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      541 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      601 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      661 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
      721 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
      781 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
      841 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
      901 gaattacaac agtactgcga
//
