LOCUS       sequence-66937-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMBD-Gate3
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(133..257)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    343..464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(354..373)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     408..427
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(737..756)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
       61 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      121 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
      181 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
      241 ttcttgtaca aagtggttcg atatcatcta gagcggccgc aggtacctga ataactaagg
      301 ccgcttccct ttagtgaggg ttaatgcttc gagcagacat gataagatac attgatgagt
      361 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      421 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      481 ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc
      541 tctacaaatg tggtaaaatc cgataaggat cgatccgggc tggcgtaata gcgaagaggc
      601 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt
      661 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      721 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttcacgccac gttcgccggc
      781 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
      841 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
      901 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
      961 caaa
//
