LOCUS       sequence-66955-e         664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.U6.miR-1306-Decoy.hPGK.GFP.WPRE
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     208..228
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_feature    449..566
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgcgccggcc ccctcgcaca gacttgtggg agaagctcgg ctactcccct gccccggtta
       61 atttgcatat aatatttcct agtaactata gaggcttaat gtgcgataaa agacagataa
      121 tctgttcttt ttaatactag ctacatttta catgataggc ttggatttct ataagagata
      181 caaatactaa attattattt taaaaaacag cacaaaagga aactcaccct aactgtaaag
      241 taattgtgtg ttttgagact ataaatatcc cttggagaaa agccttgttt gacggcgcta
      301 ggatcatcaa ccatcaccac ctcagccaac gtcaagtatt ctggtcacag aatacaacca
      361 tcaccacctc agccaacgtc aagatgatcc tagcgccgtc ttttttgaat tcgatatcaa
      421 gcttctcgag gctagtctcg tgatcgataa aattttgaat ttttgtaatt tgtttttgta
      481 attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc
      541 ccccaatccc cccttttctt ttaaaagtta accgataccg tcgagatccg ttcactaatc
      601 gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg
      661 gtcc
//
