LOCUS       sequence-66956-e         581 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.U6.miR-130b-Decoy.hPGK.GFP.WPRE
FEATURES             Location/Qualifiers
     source          1..581
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     205..225
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_feature    448..565
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gccggccccc tcgcacagac ttgtgggaga agctcggcta ctcccctgcc ccggttaatt
       61 tgcatataat atttcctagt aactatagag gcttaatgtg cgataaaaga cagataatct
      121 gttcttttta atactagcta cattttacat gataggcttg gatttctata agagatacaa
      181 atactaaatt attattttaa aaaacagcac aaaaggaaac tcaccctaac tgtaaagtaa
      241 ttgtgtgttt tgagactata aatatccctt ggagaaaagc cttgtttgac ggcgctagga
      301 tcatcaacat gccctttcaa gattgcactg caagtattct ggtcacagaa tacaacatgc
      361 cctttcaaga ttgcactgca agatgatcct agcgccgtct tttttgaatt cgatatcaag
      421 cttctcgagg ctagtctcgt gatcgataaa attttgaatt tttgtaattt gtttttgtaa
      481 ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg cactgtaccc
      541 cccaatcccc ccttttcttt taaaagttaa ccgataccgt c
//
