LOCUS       sequence-66962-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modified pcDNA3.1 vector for ligation independent cloning, including
            a ccdB cassette for eliminating no-insert background.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     pUB2.1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUB2.1
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(76..95)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
       61 gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
      121 caccgggtaa agttcacggg ggactttatc tgacagcaga cgtgcactgg ccagggggat
      181 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
      241 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt
      301 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
      361 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca
      421 gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt
      481 cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca
      541 gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt
      601 agtaagccgg atcctctaga ttacgccccg cctgccactc atcgcagtac tgttgtaatt
      661 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
      721 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
      781 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
      841 ctga
//
