LOCUS       sequence-66966-e         579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.U6.miR-16-Decoy.hPGK.GFP.WPRE
FEATURES             Location/Qualifiers
     source          1..579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..229
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_feature    452..569
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccgcgccggc cccctcgcac agacttgtgg gagaagctcg gctactcccc tgccccggtt
       61 aatttgcata taatatttcc tagtaactat agaggcttaa tgtgcgataa aagacagata
      121 atctgttctt tttaatacta gctacatttt acatgatagg cttggatttc tataagagat
      181 acaaatacta aattattatt ttaaaaaaca gcacaaaagg aaactcaccc taactgtaaa
      241 gtaattgtgt gttttgagac tataaatatc ccttggagaa aagccttgtt tgacggcgct
      301 aggatcatca accgccaata ttttggtgct gctacaagta ttctggtcac agaatacaac
      361 cgccaatatt ttggtgctgc tacaagatga tcctagcgcc gtcttttttg aattcgatat
      421 caagcttctc gaggctagtc tcgtgatcga taaaattttg aatttttgta atttgttttt
      481 gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt
      541 accccccaat cccccctttt cttttaaaag ttaaccgat
//
