LOCUS       sequence-6697-ex        2340 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM, Puigserver P
  TITLE     Nutrient control of glucose homeostasis through a complex of 
            PGC-1alpha and SIRT1.
  JOURNAL   Nature 2005 Mar 3;434(7029):113-8.
  PUBMED    15744310
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2005-03-3"; volume: "434(7029)"; pages: "113-8"
COMMENT     Sequence Label: pAd-Track Flag-SIRT1 H355A
FEATURES             Location/Qualifiers
     source          1..2340
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             49..81
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             127..2340
                     /codon_start=1
                     /product="mouse NAD+-dependent protein deacetylase 
                     sirtuin-1"
                     /label=SIRT1
                     /note="homolog of budding yeast Sir2"
                     /translation="MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGR
                     SPGEPSAAVAPAAAGCEAASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRR
                     EPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLLTDGLLTNGFHSCESDDDDRTSHA
                     SSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSE
                     PPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPD
                     LPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNID
                     TLEQVAGIQRILQCAGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIM
                     KPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINR
                     EPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLS
                     ELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQT
                     SRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFV
                     PPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLED
                     DTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS"
     regulatory      181..190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggactaca aagacgatga cgataaagca agaagagcat ctgtgcatat ggctagcatg
       61 actggtggac agcaaatggg tcgcggatcc gaattcgagc tccgtcgaca gagaggcagt
      121 tggaagatgg cggacgaggt ggcgctcgcc cttcaggccg ccggctcccc ttccgcggcg
      181 gccgccatgg aggccgcgtc gcagccggcg gacgagccgc tccgcaagag gccccgccga
      241 gacgggcctg gcctcgggcg cagcccgggc gagccgagcg cagcagtggc gccggcggcc
      301 gcggggtgtg aggcggcgag cgccgcggcc ccggcggcgc tgtggcggga ggcggcaggg
      361 gcggcggcga gcgcggagcg ggaggccccg gcgacggccg tggccgggga cggagacaat
      421 gggtccggcc tgcggcggga gccgagggcg gccgacgact tcgacgacga cgagggcgag
      481 gaggaggacg aggcggcggc ggcagcggcg gcggcagcga tcggctaccg agacaacctc
      541 ctgttgaccg atggactcct cactaatggc tttcattcct gtgaaagtga tgacgatgac
      601 agaacgtcac acgccagctc tagtgactgg actccgcggc cgcggatagg tccatatact
      661 tttgttcagc aacatctcat gattggcacc gatcctcgaa caattcttaa agatttatta
      721 ccagaaacaa ttcctccacc tgagctggat gatatgacgc tgtggcagat tgttattaat
      781 atcctttcag aaccaccaaa gcggaaaaaa agaaaagata tcaatacaat tgaagatgct
      841 gtgaagttac tgcaggagtg taaaaagata atagttctga ctggagctgg ggtttctgtc
      901 tcctgtggga ttcctgactt cagatcaaga gacggtatct atgctcgcct tgcggtggac
      961 ttcccagacc tcccagaccc tcaagccatg tttgatattg agtattttag aaaagaccca
     1021 agaccattct tcaagtttgc aaaggaaata tatcccggac agttccagcc gtctctgtgt
     1081 cacaaattca tagctttgtc agataaggaa ggaaaactac ttcgaaatta tactcaaaat
     1141 atagatacct tggagcaggt tgcaggaatc caaaggatcc ttcagtgtgc tggttccttt
     1201 gcaacagcat cttgcctgat ttgtaagtac aaagttgatt gtgaagctgt tcgtggagac
     1261 atttttaatc aggtagttcc tcggtgccct aggtgcccag ctgatgagcc acttgccatc
     1321 atgaagccag agattgtctt ctttggtgaa aacttaccag aacagtttca tagagccatg
     1381 aagtatgaca aagatgaagt tgacctcctc attgttattg gatcttctct gaaagtgaga
     1441 ccagtagcac taattccaag ttctataccc catgaagtgc ctcaaatatt aataaatagg
     1501 gaacctttgc ctcatctaca ttttgatgta gagctccttg gagactgcga tgttataatt
     1561 aatgagttgt gtcataggct aggtggtgaa tatgccaaac tttgttgtaa ccctgtaaag
     1621 ctttcagaaa ttactgaaaa acctccacgc ccacaaaagg aattggttca tttatcagag
     1681 ttgccaccaa cacctcttca tatttcggaa gactcaagtt cacctgaaag aactgtacca
     1741 caagactctt ctgtgattgc tacacttgta gaccaagcaa caaacaacaa tgttaatgat
     1801 ttagaagtat ctgaatcaag ttgtgtggaa gaaaaaccac aagaagtaca gactagtagg
     1861 aatgttgaga acattaatgt ggaaaatcca gattttaagg ctgttggttc cagtactgca
     1921 gacaaaaatg aaagaacttc agttgcagaa acagtgagaa aatgctggcc taatagactt
     1981 gcaaaggagc agattagtaa gcggcttgag ggtaatcaat acctgtttgt accaccaaat
     2041 cgttacatat tccacggtgc tgaggtatac tcagactctg aagatgacgt cttgtcctct
     2101 agttcctgtg gcagtaacag tgacagtggc acatgccaga gtccaagttt agaagaaccc
     2161 ttggaagatg aaagtgaaat tgaagaattc tacaatggct tggaagatga tacggagagg
     2221 cccgaatgtg ctggaggatc tggatttgga gctgatggag gggatcaaga ggttgttaat
     2281 gaagctatag ctacaagaca ggaattgaca gatgtaaact atccatcaga caaatcataa
//
