LOCUS       sequence-66973-e         584 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.U6.miR-195-Decoy.hPGK.GFP.WPRE
FEATURES             Location/Qualifiers
     source          1..584
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..230
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_feature    451..568
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccgcgccggc cccctcgcac agacttgtgg gagaagctcg gctactcccc tggccccggt
       61 taatttgcat ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat
      121 aatctgttct ttttaatact agctacattt tacatgatag gcttggattt ctataagaga
      181 tacaaatact aaattattat tttaaaaaac agcacaaaag gaaactcacc ctaactgtaa
      241 agtaattgtg tgttttgaga ctataaatat cccttggaga aaagccttgt ttgacggcgc
      301 taggatcatc aacgccaata tttgagtgct gctacaagta ttctggtcac agaatacaac
      361 gccaatattt gagtgctgct acaatatgat cctagcgccg tcttttttga attcgatatc
      421 aagcttctcg aggctagtct cgtgatcgat aaaattttga atttttgtaa tttgtttttg
      481 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
      541 ccccccaatc cccccttttc ttttaaaagt taaccgatac cgtc
//
