LOCUS       sequence-66980-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Finney-Manchester SP, Maheshri N
  TITLE     Harnessing mutagenic homologous recombination for targeted 
            mutagenesis in vivo by TaGTEAM.
  JOURNAL   Nucleic Acids Res. 2013 Mar 7.
  PUBMED    23470991
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Mar 7."
COMMENT     Sequence Label: PRS303-240xtetO
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    645..663
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    674..692
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    703..721
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    750..768
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    779..797
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    808..826
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 atacgactca ctatagggcg aattggagct ccaccgcggt ggcggccgct ttcaagtagt
       61 tcacaaagag actcaaactg agatcatgga gacgtcggca agcaattgat tgttagttgt
      121 tatcaaaact cattagcttc ggtaaaacta catagattga aaatcagtag taacatacaa
      181 cgtcgtttgc atggggttac aaaccgacca acctgatgct ttcaaagatt ctttacttac
      241 tggttcacag tggccaagaa gcatataaat ctgtactttg cagtaaaaaa cagatgatat
      301 tgctcattcc cagcgattgg gcgcgccatt ttgacatata ctgatatgga cctcttgttt
      361 cgtataaatc gctatttatt tccccagata actaaagaaa atccttcaac ccagcgttct
      421 atattactat attctcaacc cgccgttttc ctgctgtgcg ataattctca ttcaatacta
      481 ccatttcaaa cctagaaaag ggtgtcttta ttaaaactgt aagaaaattt tatcatgaac
      541 tctgatgaaa agtcttcgga agatcaactt tttgattttt caaaaatgaa agaaactgtt
      601 gtctccattt atggcctcat atctgaatct agagtacgtc cgtctctcta tcactgatag
      661 ggattgctag atttctctat cactgatagg gacacgtcgc cctctctatc actgataggg
      721 atggccggcc gtgctagagg ggcggtgctt ctctatcact gatagggact ttcactcttc
      781 tctatcactg atagggatga tcaacgttct ctatcactga tagggatccc cactcttgct
      841 agagtggtct cacctctcta tcactgacag ggacctctct tcctctctat ca
//
