LOCUS       sequence-66985-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX Grb7-SH2
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..37
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(496..518)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(663..681)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        749..853
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catctcaatg gatctggaag ttctgttcca ggggcccctg ggatccgggc gcgccatgtc
       61 cggcacgagc ctcagtgcag ccatccaccg cacccaactc tggttccacg ggcgcatttc
      121 ccgtgaggag agccagcggc ttattggaca gcagggcttg gtagacggcc tgttcctggt
      181 ccgggagagt cagcggaacc cccagggctt tgtcctctct ttgtgccacc tgcagaaagt
      241 gaagcattat ctcatcctgc cgagcgagga ggagggccgc ctgtacttca gcatggatga
      301 tggccagacc cgcttcactg acctgctgca gctcgtggag ttccaccagc tgaaccgcgg
      361 catcctgccg tgcttgctgc gccattgctg cacgcgggtg gccctcttaa ttaacgaatt
      421 cccgggtcga ctcgagcggc cgcatcgtga ctgactgacg atctgcctcg cgcgtttcgg
      481 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      541 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      601 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tataattctt gaagacgaaa
      661 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
      721 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      781 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      841 aaaaaggaag agtatgagta ttcaacattt ccgtgtcncc cttattccct tttttgcggc
      901 atttgccttc c
//
