LOCUS       sequence-66997-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Finney-Manchester SP, Maheshri N
  TITLE     Harnessing mutagenic homologous recombination for targeted 
            mutagenesis in vivo by TaGTEAM.
  JOURNAL   Nucleic Acids Res. 2013 Mar 7.
  PUBMED    23470991
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Mar 7."
COMMENT     Sequence Label: PRS415-Gal1pr-sctetR-FokI
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..629
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
     primer_bind     complement(85..104)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 aaaaacgcaa agtgtctaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc
       61 ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag cttggtgtag
      121 agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca
      181 ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa agctggcaag
      241 attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag
      301 caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat
      361 tagccttttt atgccaacaa ggtttttcac tagagaacgc gttatatgca ctcagcgctg
      421 tggggcattt taccttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag
      481 aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct atcgaattat
      541 ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat
      601 tagaaaaaca acttaaatgt gaaagtgggg actcaggagg cggtggatcc ggtggaggcg
      661 gttctggcgg tggaggctct ggaggtggcg gatcgggcgg aggtggctct agatctcgtc
      721 tggacaagag caaagtcata aactctgctc tggaattact caatgaagtc ggtatcgaag
      781 gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc ctgtactggc
      841 acgtgaagaa caagcgggcc ctgctcgatg ccctggcaat cgagatgctg gacaggcatc
      901 atacccactt ctgccccctg gaagg
//
