LOCUS       sequence-66999-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX Cis1-SH2
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..28
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(497..519)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(664..682)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggatctggaa gttctgttcc aggggcccct gggatccata gccaagacct tctcctacct
       61 tcgggaatct ggctggtatt ggggttccat tacggccagc gaggcccgac aacacctgca
      121 gaagatgcca gaaggcacgt tcttagtacg tgacagcacg caccccagct acctgttcac
      181 gctgtcagtg aaaaccactc gtggccccac caatgtacgc attgagtatg ccgactccag
      241 cttccgtctg gactccaact gcttgtccag gccacgcatc ctggcctttc cggatgtggt
      301 cagccttgtg cagcactatg tggcctcctg cactgctgat acccgaagcg acagccccga
      361 tcctgctccc accccggccc tgcctatgcc taaggaggat gcgcctagtg acccagcact
      421 gcctggaatt ctatgagcgg ccgcatcgtg actgactgac gatctgcctc gcgcgtttcg
      481 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
      541 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
      601 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtataattct tgaagacgaa
      661 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      721 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaa
//
