LOCUS       sequence-67000-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX Crk-SH2
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             436..480
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(544..566)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(711..729)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catctcaatg gatctggagt tctgttccag gggcccctgg gatccatggc gggcaacttc
       61 gactcggagg agcggagtag ctggtactgg gggcggttga gtcggcagga ggcggtggcg
      121 ctgctgcagg gccagcggca cggggtgttc ctggtgcggg actcgagcac cagccccggg
      181 gactatgtgc tcagcgtctc agagaactcg cgcgtctccc actacatcat caacagcagc
      241 ggcccgcgcc cgccggtgcc accgtcgccc gcccagcctc cgcccggggt gagcccctcc
      301 agactccgaa taggagatca agagtttgat tcattgcctg ctttactgga attctacaaa
      361 atacactatt tggacactac aacgttgata gaaccagttt ccagatccag gcagggtgga
      421 agcttgcttg gtggcggtct gaacgacatc ttcgaggctc agaaaatcga atggcacgaa
      481 taagcggccg catcgtgact gactgacgat ctgcctcgcg cgtttcggtg atgacggtga
      541 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
      601 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
      661 gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg gcctcgtgat
      721 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      781 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      841 gtatccgctc atga
//
