LOCUS       sequence-67006-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX FynA-SH2
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..36
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             409..453
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(517..539)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(684..702)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        770..874
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcaaatcgg atctggaagt tctgttccag gggcccctgg gatccgactc tatccaggca
       61 gaagagtggt actttggaaa acttggccga aaagatgctg agcgacagct attgtccttt
      121 ggaaacccaa gaggtacctt tcttatccgc gagagtgaaa ccaccaaagg tgcctattca
      181 ctttctatcc gtgattggga tgatatgaaa ggagaccatg tcaaacatta taaaattcgc
      241 aaacttgaca atggtggata ctacattacc acccgggccc agtttgaaac acttcagcag
      301 cttgtacaac attactcaga gagagctgca ggtctctgct gccgcctagt agttccctgt
      361 cacaaaggga tgccaggaat tcatcgtgac gcaagcttgc ttggtggcgg tctgaacgac
      421 atcttcgagg ctcagaaaat cgaatggcac gaataagcgg ccgcatcgtg actgactgac
      481 gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
      541 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
      601 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
      661 gtataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      721 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      781 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      841 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcacattt c
//
