LOCUS       sequence-67008-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX FynT-SH2
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             401..445
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(509..531)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(676..694)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        762..866
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcaatggac tggagttctg ttccggggcc cctgggatcc gactctatcc aggcagaaga
       61 gtggtacttt ggaaaacttg gccgaaaaga tgctgagcga cagctattgt cctttggaaa
      121 cccaagaggt acctttctta tccgcgagag tgaaaccacc aaaggtgcct attcactttc
      181 tatccgtgat tgggatgata tgaaaggaga ccatgtcaaa cattataaaa ttcgcaaact
      241 tgacaatggt ggatactaca ttaccacccg ggcccagttt gaaacacttc agcagcttgt
      301 acaacattac tcagagaaag ctgatggttt gtgttttaac ttaactgtga ttgcatcgag
      361 ttgtaccgga attcatcgtg acgcaagctt gcttggtggc ggtctgaacg acatcttcga
      421 ggctcagaaa atcgaatggc acgaataagc ggccgcatcg tgactgactg acgatctgcc
      481 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
      541 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      601 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtataatt
      661 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      721 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      781 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      841 ttcaataata ttgaaaaagg aagagtatga gtattcactt ctgccctatc cttt
//
