LOCUS       sequence-67051-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMBD-Gate4
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    43..167
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(208..513)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(310..329)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gccggaattc ccggggatcc gtcgacttga cgcgttgata tcaccacttt gtacaagaaa
       61 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
      121 aaacagacta cataatactg taaaacacaa catatccagt cactatggtc gacctgcaga
      181 ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt
      241 tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg
      301 cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg
      361 gtaaagttca cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat
      421 ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct
      481 ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcagcaa
      541 aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt
      601 tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg
      661 agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt
      721 aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat atcagtatat
      781 attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag
      841 ccggatccac gcgtttacgc cccgccctgc cactcatcgc a
//
