LOCUS       sequence-67052-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMBD-Gate4
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(102..108,109..126,127..132))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(157..281)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    365..486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(376..395)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     430..449
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(759..778)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
       61 agctcctgaa aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc
      121 ataaagtgta aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt
      181 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
      241 tcattttacg tttctcgttc agcttttttg tacaaacttg tgatatcatc tagagcggcc
      301 gcaggtacct gaataactaa ggccgcttcc ctttagtgag ggttaatgct tcgagcagac
      361 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
      421 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
      481 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
      541 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tccgataagg atcgatccgg
      601 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
      661 atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      721 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      781 ctttcacgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      841 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      901 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg a
//
