LOCUS       sequence-67053-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMBD-Gate5
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..535
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    578..702
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(845..864)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
       61 taggctagcc agcttgaagc aagcctcctg aaagatgaag ctactgtctt ctatcgaaca
      121 agcatgcgat atttgccgac ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc
      181 caagtgtctg aagaacaact gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct
      241 gactagggca catctgacag aagtggaatc aaggctagaa agactggaac agctatttct
      301 actgattttt cctcgagaag accttgacat gattttgaaa atggattctt tacaggatat
      361 aaaagcattg ttaacaggat tatttgtaca agataatgtg aataaagatg ccgtcacaga
      421 tagattggct tcagtggaga ctgatatgcc tctaacattg agacagcata gaataagtgc
      481 gacatcatca tcggaagaga gtagtaacaa aggtcaaaga cagttgactg tatcgccgga
      541 attcccgggg atccgtcgac ttgacgcgtt gatatcaacc actttgtaca agaaagctga
      601 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      661 gactacataa tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc
      721 tgtgtataag ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga
      781 tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac
      841 tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa
      901 gttcacggga gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc
      961 gcccgggcgt gtcaataata tcactctgta catccacaa
//
