LOCUS       sequence-67054-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMBD-Gate5
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    44..168
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(209..514)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(311..330)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gccggaattc ccggggatcc gtcgacttga cgcgttgata tcaaccactt tgtacaagaa
       61 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
      121 aaaacagact acataatact gtaaaacaca acatatccag tcactatggt cgacctgcag
      181 actggctgtg tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt
      241 ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg
      301 gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg
      361 ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca
      421 tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc
      481 tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca
      541 aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct
      601 ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg
      661 gagatattga catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg
      721 taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata
      781 tattcttata ccgcaaaaat cagcgcgc
//
